Research:
Using PIV5 as a Vector for Vaccine Development

PIV5 is an excellent vector for expressing foreign genes and for live vaccine development. PIV5 does not have a DNA phase in its life cycle and it replicates solely in cytoplasm, therefore using PIV5 as a vector avoids possible unintended consequences from genetic modifications of host cell DNAs. The genome structure of PIV5 is stable and simple. Using the reverse genetics system for the PIV5, a recombinant PIV5 containing a hemaglutinin (HA) gene from influenza A virus (rPIV5-HA) was generated. In collaboration with Dr. Durbin at the Ohio State University, we found a single dose inoculation of the virus provided immunity to mice against influenza A virus infection.

Recombinant PIV5 viruses expressing other viral proteins such as gag protein of HIV have been generated. The virus grows normally as wild type PIV5. At present, the virus is being tested as a live vaccine in mice.